The amount of resveratrol in a bottle of red wine varies from 2 mg to 14 mg, mostly on the lower side. Dietary supplements of resveratrol are typically derived from the roots of Japanese knotweed (Polygonum cuspidatum), a far more economical source than grapes. Doses will range from a basic protective dose of a few milligrams (like a bottle of red wine), up to 100 mg per serving or more (a therapeutic dose). It is readily absorbed, reaching peak blood levels in 30 minutes, and then rather rapidly cleared by your liver. Thus, it is better to spread out intake during the day than take a large amount all at once.

At this point, other than the colorful history and longevity benefits associated with red wine consumption, the majority of the extensive resveratrol research has been carried out with cell studies and small animals. The implications of this research are mind-boggling, clearly showing significant extension of life span.

SIRT1 first drew attention as the primary gene signal involved with the longevity benefits of calorie restriction. A very simple explanation is that when you are in a food scarcity situation, SIRT1 is activated so as to help break down your stored fat to use as fuel as well as to boost up your energy so that you have enough energy to hunt for new food. SIRT1 is part of a famine-related survival system.

Many experiments with animals show that by restricting calorie intake SIRT1 is naturally activated, a finding that goes along with a noticeably extended lifespan, better fat and cholesterol metabolism, more efficient immune function, and better cardiovascular health. A number of humans have taken up calorie restriction experiments on themselves, and pictures of them do not portray the portrait of health. In fact, you would be hard pressed to pick out of a line-up someone on a self-induced calorie restriction diet and someone coming in for anorexia treatment. Which gets to my point, what is the difference between a calorie restriction diet and anorexia?

REFERENCE : Modified article from http://www.wellness resources. com/tips/ articles/

Resveratrol and quercetin, the primary small molecules under study, concentrated in red wine and also found in other botanical sources, exhibit the wide range of biological activity researchers are searching for.  The biological action of resveratrol is so profoundly broad that one group of researchers said: “Ask not what resveratrol can do, ask what it cannot do.”  Researchers at the University of Illinois state that “resveratrol holds great promise for future development as a chemopreventive agent that may be useful for several disorders.”  [Annals N Y Academy Sciences 2002 May; 957: 210-29]  Another research report describes resveratrol’s effects as so overwhelming that it exerts “a whiff that induces a biologically specific tsunami.” [Cancer Biology Therapy 2004 Sep; 3(9):889-90]
So the curious public can gain some insight into the enthusiasm medical researchers have for red wine molecules, the following chart has been produced to document some of the remarkable biological activity of resveratrol.  Here is the list:

THE MANY BIOLOGICAL ACTIONS OF RESVERATROL

Biological action	Reference
Preserves or stimulates superoxide dismutase (antioxidant)	J Agriculture Food Chemistry 2005 May 18; 53(10):4182-6; Free Radical Biology Medicine 2003 Apr 1; 34(7):810-7
Elevates glutathione (antioxidant)	J Agric Food Chem. 2005 May 18; 53 (10):4182-6; Archives Biochemistry Biophysics 2000 Sep 15; 381 (2):253-63
Elevates catalase activity (antioxidant)	Life Science 2003 May 2; 72 (24):2741-50
Prolongs life of cells via Sirtuin 1 gene	Trends Pharmacological Sciences 2005; 26: 94-103
Anti-histamine action (allergy)	Planta Medica 2004 Apr;70(4):305-9.
Anti-inflammatory agent	J Chemotherapy 2004 Nov;16 Suppl 4:3-6; J Environ Pathology Toxicology Oncology 2004; 23(3):215-26
COX-2 inhibitor (anti-inflammatory)	Inflammation Research 2005 Apr; 54(4):158-62
Potentially helpful for rheumatoid arthritis	International Immunopharmacology 2005 May;5(5):849-56
Inhibits pancreatitis	World J Gastroenterology 11: 3171-74, 2005
Promotes DNA repair (via Sirtuin 1 gene)	Nature 2003 Sep 11; 425 (6954):191-6
Inhibits abnormal new blood vessel formation (angiogenesis)	J Physiology Pharmacology 2005 Mar;56 Suppl 1:51-69
Inhibits dietary sugar absorption	Journal Natural Products 2001; 64: 381-84
Regulates blood sugar	J Agric Food Chemistry 2005 May 4; 53(9):3403-7
Normalizes blood pressure	J Hypertension 2000 Dec; 18(12):1833-40
ACE Inhibitor properties	European J Pharmacology 2005 May 16; 515(1-3):1-9
Prevents blood clots	Blood Coagulation Fibrinolysis 2004 Sep;15(6):441-6
Reduces LDL cholesterol	Life Sciences 2003 Aug 1; 73(11):1393-400
Reduces triglycerides	Life Sciences 2003 Aug 1; 73(11):1393-400
Raises HDL “good” cholesterol	J Agric Food Chemistry 2005 May 4; 53(9):3403-7
Calms effects of estrogen (estrogen blocker)	J Steroid Biochemical Mol Biology 2005 Apr;94(5):431-43
Prevents bone loss	J Medicinal Food 2005 Spring;8(1):14-9
Protects retinal cells (retinal pigment epithelium)	Chemical Biological Interaction 2005 Jan 15;151(2):143-9
Increases sperm count	J Nutrition 2005 Apr;135(4):757-60
Inhibits viral growth (influenza)	J Infectious Disease 2005 May 15; 191 (10):1719-29
Inhibits viral growth (HIV)	J Pharm Science 2004 Oct; 93 (10):2448-57
Inhibits viral growth (herpes)	Antiviral Research 2004 Jan;61(1):19-26; 1999 Oct;43(3):145-55
Antibiotic against bacteria (Chlamydia)	Atherosclerosis 2003 Dec;171(2):379-80
Antibiotic against bacteria (H. pylori)	Am J Gastroenterology 2003 Jun;98(6):1440-1
Inhibits growth of fungi (yeast, mold)	J Agriculture Food Chemistry 2003 Feb 26;51(5):1464-8
Inhibits initiation of tumors	Proc Natl Sci Counc Repub China B. 1999 Jul;23(3):99-106
Inhibits growth of tumors	Drugs Exp Clin Res. 1999;25(2-3):65-77.
Inhibits spread of tumors	Proc Natl Sci Council Repub China B. 1999 Jul;23(3):99-106
Protects brain cells	Ann N Y Academy Sciences 2003 May;993:276-86
Inhibits hepatitis (liver inflammation)	Hepatogastroenterology 2002 Jul-Aug;49(46):1102-8
Eradicates plaque in brain (beta amyloid toxicity)	British J Pharmacology 2004 Mar;141 (6):997-1005
Chelates metals (copper)	Biochem Pharmacology 1997 May 9; 53(9):1347-55
Anti-leukemia agent	Leukemia Lymphoma 2002 May;43(5):983-7
Inhibits prostate cancer	Cancer Epidemiology Biomarkers Prevention 2005 Mar;14(3):596-604
Inhibits breast cancer	Molecular Nutrition Food Research 2005 May;49(5):462-71
Inhibits melanoma	Neoplasia 2005 Jan;7(1):37-47
Inhibits liver cancer	World J Gastroenterology 2003 Oct;9(10):2341-3
Inhibits brain cancer	Molecular Cancer Therapy 2005 Apr;4(4):554-61
Inhibits pancreatic cancer	Pancreas 2002 Nov;25(4):e71-6
Inhibits kidney cancer	Cancer Biology Therapy 2004 Sep; 3(9):882-8
Inhibits colon cancer	International J Cancer. 2005 Jun 10;115(2):194-201
Inhibits ovarian and cervical cancer	Anticancer Res. 2004 Sep-Oct;24(5A):2783-840.
Inhibits lymphoma	Cancer Letters 2001 Feb 10;163(1):43-9
Non-toxic	J Nutrition 2002 Feb;132(2):257-60

The accuracy and reliability of the test results and interpretation is based directly upon the laboratory receiving a properly collected hair sample that is clean and free from external contaminants. It is difficult for the laboratory to make a determination as to whether the sample was taken properly. Accordingly, the laboratory assumes no responsibility for results from an improperly submitted hair sample. C Test results – The ideal values for minerals are indicated by the gray bands, i.e. calcium 40 mgs%, magnesium 6 mgs%, sodium 25 mgs%, potassium 10 mgs%, etc. Your mineral values are printed directly above the name of each mineral. The black rectangle blocks represent a bar graph showing where your values lie in relation to the ideal values. Significant mineral ratios and your oxidation rate are located at the bottom of the graph. C Reference ranges (blue shaded area) indicated on the graph of test results represent statistical “ideal” levels.

These reference ranges should not be considered as absolute in considering mineral excesses, deficiencies or toxic levels of elements. C The results of the hair tissue mineral analysis are reported in milligrams percent (mg%) or milligrams per 100 grams of hair. C Accutrace Laboratories, Inc., a wholly owned subsidiary of Analytical Research Laboratories, Inc., automatically retests any mineral levels that are found to be outside an expected range of results, provided enough hair is available for this process. C Test results were obtained using sophisticated ICP-MS instrumentation and procedures in a clinical laboratory environment with government regulatory standards outlined by the Department of Health and Human Services under the Clinical Laboratory Improvement Amendment (CLIA).

The interpretation of your hair tissue mineral analysis depends upon developing a “metabolic blueprint” of how the body is responding to stress. The ability to determine the stage of stress (42) and the oxidation rate (48) from a hair tissue mineral analysis makes it possible to assess the likelihood of many conditions and guide correction based upon your metabolic imbalances. A thorough interpretation of the tests results also requires the identification of mineral levels, ratios and metabolic patterns.

METABOLIC PATTERNS
A metabolic pattern is a combination of mineral levels and/or mineral ratios that reveal how the body is responding to stress. Identifying metabolic patterns simplify the interpretation as the science of mineral balancing is almost always aimed at improving major metabolic patterns and not a single mineral. A general rule is that metabolic patterns are the most important factors to consider when interpreting a hair tissue mineral analysis, followed by mineral ratios and mineral levels. Ratios represent mineral relationships and balances in the body.

OXIDATION RATE
The term “oxidation rate” refers to the “burning” of foods in the body or how the body converts the foods you eat to energy. There are three types of oxidation rates, slow oxidation, fast oxidation and mixed oxidation. There are varying degrees of each oxidation rate and ideally it would be best to have either a slightly slow or slightly fast oxidation rate.

TOXIC METALS AND CHEMICALS
The presence of toxic metals and chemicals can potentially present a serious health hazard. (1, 11,12,13,14,15,16,19,22,29,31,39,51). A serious problem today is that a large number of babies are born high in toxic metals due to toxicity in the mothers. A review of over 400 medical studies by the US Environmental Protection Agency revealed that hair tissue mineral analysis is a meaningful test to detect toxic metals (47). Toxic metals can cause hundreds of symptoms and contribute to many serious health conditions. There are no safe levels of toxic metals and reducing the presence of toxic metals is a primary goal of your nutritional balancing program. Seven different methods are used simultaneously in your recommended dietary, supplement and lifestyle program to assist in the reduction of toxic metals. These are 1) improve your energy level, 2) provide support for the organs of elimination, 3) inhibit the sympathetic nervous system, 4) reduce exposure, 5) supplement with heavy metal antagonists, 6) supplement with natural heavy metal chelators and 7) recommend other natural detoxification methods.

The hair tissue mineral analysis does not test for toxic chemicals such as pesticides and solvents. However, enhancing energy production, inhibiting the sympathetic nervous system, assisting the organs of elimination and reducing exposure to all toxins greatly assists the removal of toxic chemicals from the body. Hair tissue mineral analysis only detects metals present in the hair tissue. No test can detect all toxic metals, as some are hidden deep within other tissues or organs. The unique value of hair mineral tissue mineral analysis is not so much to detect toxic metals, but to guide the balancing of body chemistry to assure their safe and swift removal. When the seven methods above are combined, the metals will be removed without the need for synthetic chelators. Toxic metals are often layered deep within body tissues. The recommended diet, supplement, lifestyle and detoxification program will slowly release layer after layer. Hidden metals will often be revealed on future mineral tests as they are eliminated through the hair, skin and through other routes.

REFERENCES & RESOURCES
1. Albrecht, W.A, The Albrecht Papers, Acres U.S.A., 1975. 2. Andersen, B.D.,The Rhythms of Nature, Harmonic Spiral, 1999. 3. Atkins, R.C., The Atkins Health Revolution, Houghton Mifflin Co., 1988. 4. Bernard, C., An Introduction to the Study of Experimental Medicine, Collier Books, 1961. 5. Bland, J., Hair Tissue Mineral Analysis, An Emergent Diagnostic Technique, Thorsons Publishing, 1984 6. Braunwald, E. Eet al, ed., Harrison’s Principles of Internal Medicine, 15th edition, McGraw-Hill, 2001 7. Crook, W.G., The Yeast Connection Handbook, Professional Books, 1999. 8. Davies, I.J.T., The Clinical Significance of the Essential Biological Metals, C.C. Thomas, 1972. 9. Douglass, W.C., The Milk of Human Kindness is Not Pasteurized, Copple House Books, 1985. 10. Douglass, W.C., Into the Light, Second Opinion Publishing, 1993. 11. Eck, P.C., Healthview Newsletter, Interview #27-29, Healthview, 1981. 12. Eck, P.C. and Wilson, L., Toxic Metals in Human Health and Disease, Eck Institute of Applied Nutrition and Bioenergetics, Ltd.,1989. 13. Casdorph, H.R. and Walker, M., Toxic Metal Syndrome, Avery Publishing, 1995. 14. Chatsworth, L. and Chatsworth, C., Energy, Healthview, 1985. 15. Cleave, T.L, The Saccharine Disease, Keats Publishing, 1975. 16. Droesti, I. and Smith, R., Neurobiology of the Trace Elements, Volumes I and II., Humana Press, 1983. 17. Gerson, M., A Cancer Therapy – Results of 50 Cases, 3rd edition, Totality Books, 1977. 18. Gittleman, A.L., Why Am I Always So Tired, Harper San Francisco, 1999. 19. Goyer, R.A. et al, Medical Toxicology, Academic Press, 1995. 20. Guyton, A.,Textbook of Medical Physiology, W.B. Saunders Co.,1995. 21. Hall, R.H., Food For Naught, The Decline in Nutrition, Vintage Books, 1974. 22. Hoffer, A. and Walker, M., Orthomolecular Nutrition, Keats Publishing, 1978 23. Jensen, B., The Chemistry of Man, 1983. 24. Kelley, W.D., One Answer to Cancer, 1980. 25. Kervan, C.L., Biological Transmutations, Beekman Publishers, 1980. 26. Kirschmann, J.D., Nutrition Almanac, McGraw-Hill , 1979. 27. Koch, W., The Survival Factor in Neoplastic and Viral Diseases, 1961. 28. Kutsky, R., Handbook of Vitamins, Minerals and Hormones, 2nd edition, Van Nostrand Reinhold, 1981. 29. Leek, R., Hair Analysis, R. Leek, 1980. 30. Ott, J.N., Health and Light, The Effects of Natural and Artificial Light on Man and Other Living Things, Pocket Books, 1976. 31. Passwater, R.A. and Cranton, E.M., Trace Minerals, Hair Analysis and Nutrition, Keats Publishing, 1983. 32. Pauling, L., Vitamin C, The Common Cold and the Flu, W.H. Freeman and Co., 1976. 33. Page, M., Degeneration-Regeneration, Nutritional Development, 1980. 34. Pearson, D. and Shaw, S., Life Extension, Warner Books, 1983. 35. Pfeiffer, C.C., Mental and Elemental Nutrients, Keats Publishing, 1975. 36. Pfeiffer, C.C., Zinc and other Micronutrients, Keats Publishing, 1978. 37. Price, W., Nutrition and Physical Degeneration, Price-Pottenger Nutrition Foundation, 1945, 1979. 38. Rapp, D.J., Is This Your Child’s World?, Bantam Books, 1996. 39. Rogers, S., Detoxify or Die, Sand Key Company, 2002. 40. Schroeder, H., The Trace Elements and Man, Devin-Adair Company, 1975. 41. Scogna, J.R., The Promethian, LEP Publications, 1983. 42. Selye, H., The Stress of Life, McGraw-Hill , 1956. 43. Schmidt, M.A., Smith, L.H. and Sehnert, K.W., Beyond Antibiotics, Healthier Options for Families, North Atlantic Books, 1993. 44. Schutte, K.H. and Myers, J.A., Metabolic Aspects of Health, Discovery Press, 1979. 45. Smith, E. et al., Principles of Biochemistry, Vols. I and II, 2nd edition, McGraw-Hill, 1978. 46. Stryer, L., Biochemistry, 2nd edition, W.H. Freeman and Company, 1981. 47. United States Environmental Protection Agency, Toxic Trace Metals in Mammalian Hair and Nails, EPA-600 4.79-049, August 1979. 48. Watson, G., Nutrition and Your Mind, Bantam books, 1972. 49. Watson, G., Personality Strength and Psycho-Chemical Energy, Harper and Row, 1979. 50. Williams, R.J., Nutrition Against Disease, Environmental Protection, Pitman Publishing, 1971. 51. Wilson, L., Nutritional Balancing and Hair Mineral Analysis, L.D. Wilson Consultants, Inc., 1998, 2005, 2010, 2014. 52. Wilson, L., Manual of Sauna Therapy, L.D. Wilson Consultants, Inc., 2003. 53. Wilson, L., Healing Ourselves, L.D. Wilson Consultants, Inc. 1995, 2000, 2003, 2007.

• Antioxidant
• Detoxifying
  • Binds to toxins to facilitate removal
  • Uses enzyme Glutathione-s-transferase (GST)
    • Mercury
    • Mycotoxins
• Cell Signal
• Immune cells

Glutathione is made up of 3 amino acids :

Cysteine (contains sulfur molecule)
Glycine
Glutamine

Liposomal Glutathione  prevents Ox – LDL formation which occurs with low GSH (reduced glutathione)

OxLDL → Foam Cells → Plaque
Rupture → Sudden Obstruction of blood vessel

Anti-oxidant and anti-atherogenic properties of liposomal glutathione: studies in vitro, and in the atherosclerotic apolipoprotein E-deficient mice. Atherosclerosis. 2007;195(2):e61-8.

• Low Glutathione in the prefronatal cortex at postmortem in individuals with Major depressive disorder, bipolar disorder and schizophrenia
• Gawryluk JW, et al Decreased levels of glutathione, the major brain antioxidant, in post-mortem prefrontal cortex from patients with psychiatric disorders. The international journal of neuropsychopharmacology 2010:1-8. PubMed: 20633320
• S-nitrosoglutathione GSNO ← GSH + NO
• formation of GSNO is a mechanism for storage and transport of nitric oxide.
• A major source of bronchodilatory NO activity
• Decreased GSNO activity found in asthma
• Protection from experimental asthma by an endogenous bronchodilator.
• Science. 2005 Jun 10;308(5728):1618-21. PMID: 15919956

I.V. Protocol for Glutathione

• Push
• Safe
• Dosage: 500-2500 mg
• A mix in 5-10 ml of sterile water
• Push over 3-5 minutes
• Repeat 2-3 times per week for the first month, then weekly, for a month, the two weekly as desired

Encourage foods that are high glutathione precursors

• Avocados
• Potato
• Strawberries
• Watermelon
• NAC
• BCAA
• Rosemary (increase glutathione transferase by 400%)